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	Provedor de dados BJMBR (2) Braz. J. Genet. (1) Genet. Mol. Biol. (1) Autor Candido-Silva,J.A. (1) Dessen,Eliana M.B. (1) Monesi,N. (1) Ramirez,Corolla C.L. (1) Rohde,Claudia (1) Sauaia,H. (1) Valente,Vera Lúcia S. (1) Viana,J.C. (1) de Almeida,J.C. (1) Mais... Palavra-chave Polytene chromosomes (4) Sciaridae (2) Anopheles bellator (1) Anopheles cruzii (1) Bradysia hygida (1) BrdUrd (1) Cytogenetic (1) DNA puffs (1) Drosophila willistoni (1) Eukaryotic Elongation Factor 1A (1) Gene amplification (1) Inversions (1) Mosquito (1) Salivary glands (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Ano 2012 (1) 2010 (2) 1996 (1) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última 5-Bromo-2’-deoxyuridine induces visible morphological alteration in the DNA puffs of the anterior salivary gland region of Bradysia hygida (Diptera, Sciaridae) BJMBR de Almeida,J.C.; Sauaia,H.; Viana,J.C.. 5-Bromo-2’-deoxyuridine (BrdUrd) has long been known to interfere with cell differentiation. We found that treatment ofBradysia hygida larvae with BrdUrd during DNA puff anlage formation in the polytene chromosomes of the salivary gland S1 region noticeably affects anlage morphology. However, it does not affect subsequent metamorphosis to the adult stage. The chromatin of the chromosomal sites that would normally form DNA puffs remains very compact and DNA puff expansion does not occur with administration of 4 to 8 mM BrdUrd. Injection of BrdUrd at different ages provoked a gradient of compaction of the DNA puff chromatin, leading to the formation of very small to almost normal puffs. By immunodetection, we show that the analogue is preferentially... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA puffs; BrdUrd; Gene amplification; Polytene chromosomes; Salivary glands; Sciaridae. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200002 Bradysia hygida (Diptera, Sciaridae) presents two eukaryotic Elongation Factor 1A gene homologues: partial characterization of the eukaryotic Elongation Factor 1A-F1 gene BJMBR Candido-Silva,J.A.; Monesi,N.. Elongation factor 1A is a highly conserved protein that participates in translation. We report the occurrence of two genes homologous to the eukaryotic Elongation Factor 1A in Bradysia hygida and describe the partial cloning and characterization of the B. hygida eukaryotic Elongation Factor 1A-F1 (BheEF1A-F1) gene. The pattern of BheEF1A-F1 expression in the salivary gland at the end of the fourth larval instar was investigated using real-time PCR. The results showed that BheEF1A-F1 expression levels are relatively constant at the time when rapid changes in protein synthesis occur in this tissue. In situ hybridization experiments coupled to Southern blot analyses showed that the BheEF1A-F1 gene is located at position 3d of the A chromosome and a second... Tipo: Info:eu-repo/semantics/article Palavras-chave: Eukaryotic Elongation Factor 1A; Bradysia hygida; Polytene chromosomes; Sciaridae. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500004 The polytene chromosomes of the mosquito Anopheles bellator compared with those of Anopheles cruzii Braz. J. Genet. Ramirez,Corolla C.L.; Dessen,Eliana M.B.. A photographic map was made of polytene chromosomes of ovarian nurse cells of Anopheles bellator females. The chromosomes of this species have complete or partial homology with those of A. cruzii, mainly in the telomeric and centromeric regions. Variability at the single band level was observed as asymmetric bands at seven different positions. One inversion (3Ra) was detected in the 3R arm. Tipo: Info:eu-repo/semantics/article Palavras-chave: Polytene chromosomes; Mosquito; Anopheles bellator; Anopheles cruzii. Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400003 Three decades of studies on chromosomal polymorphism of Drosophila willistoni and description of fifty different rearrangements Genet. Mol. Biol. Rohde,Claudia; Valente,Vera Lúcia S.. Drosophila willistoni (Insecta, Diptera) is considered a paradigm for evolutionary studies. Their chromosomes are characterized by multiple paracentric inversions that make it hard to identify and describe chromosomal poly-morphisms. In the present report we attempted to systematize the description of all the 50 inversions found in the last three decades, since we have been studying the chromosomes of several individuals of 30 different populations, including the one used in the genome sequencing project (Gd-H4-1). We present the photographic register of 11 arrangements in the left arm of the X chromosome (XL), eight in the right arm (XR), 10 in the left arm of chromosome II (IIL), eight in its right arm (IIR) and 13 in chromosome III. This information... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetic; Polytene chromosomes; Inversions; Drosophila willistoni. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600012 Registros recuperados: 4 Primeira ... 1 ... Última

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